KRT14 Antikörper (AA 351-472)

Produktnummer ABIN6939908

Dieses Anti-KRT14-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT14 in IHC, FACS und StM. Geeignet für Human, Ratte, Maus und Schwein.

Kurzübersicht für KRT14 Antikörper (AA 351-472) (ABIN6939908)

Target: KRT14 (Keratin 14 (KRT14))

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Klon: KRT14-2375

Produktdetails anti-KRT14 Antikörper

Bindungsspezifität: AA 351-472

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human KRT14 fragment (around aa351-472) (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 or HeLa cells. Skin or Squamous Cell Carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: KRT14

Hintergrund: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Molekulargewicht: 50kDa

Gen-ID: 3861

UniProt: P02533