Keratin 10 Antikörper

Produktnummer ABIN6939898

Dieses Anti-Keratin 10-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Keratin 10 in IHC, FACS, IF und StM. Geeignet für Human und Maus.

Kurzübersicht für Keratin 10 Antikörper (ABIN6939898)

Target: Keratin 10 (KRT10)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Klon: AE20

Produktdetails anti-Keratin 10 Antikörper

Spezifität: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Aufreinigung: Purified by Protein A/G

Immunogen: Human cytokeratin 10

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 10

Target: Keratin 10 (KRT10)

Andere Bezeichnung: KRT10

Molekulargewicht: 56.5kDa

Gen-ID: 3858

UniProt: P13645