Rekombinanter KRT8 Antikörper

Produktnummer ABIN6939892

Der Kaninchen Monoklonal Anti-KRT8-Antikörper wurde für IHC, StM und ISt validiert. Er ist geeignet, KRT8 in Proben von Human, Rind (Kuh), Kaninchen, Schwein und Schaf zu detektieren.

Kurzübersicht für Rekombinanter KRT8 Antikörper (ABIN6939892)

Target: KRT8 (Keratin 8 (KRT8))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Rind (Kuh), Kaninchen, Schwein, Schaf

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser KRT8 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)

Klon: KRT8-2174R

Produktdetails anti-KRT8 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human KRT8 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu KRT8

Target: KRT8 (Keratin 8 (KRT8))

Andere Bezeichnung: KRT8

Hintergrund: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.

Molekulargewicht: 52.5kDa

Gen-ID: 3856

UniProt: P05787