ARF1 Antikörper (C-Term)

Produktnummer ABIN6939845

Dieses Anti-ARF1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ARF1 in WB, ELISA, IHC, IF, StM und Coat. Geeignet für Human, Ratte und Maus.

Kurzübersicht für ARF1 Antikörper (C-Term) (ABIN6939845)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)

Klon: 3F1

Produktdetails anti-ARF1 Antikörper

Bindungsspezifität: AA 174-180, C-Term

Aufreinigung: Purified by Protein A/G

Immunogen: Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Kidney, Liver, Brain or Breast.

Known Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Andere Bezeichnung: ARF1

Hintergrund: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60 % sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Molekulargewicht: 20kDa

Gen-ID: 375

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process