ICAM1 Antikörper

Produktnummer ABIN6939678

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ICAM1 in FACS, IHC, IF, Func und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ICAM1 Antikörper (ABIN6939678)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Staining Methods (StM)

Klon: W-CAM-1-same as Wehi-CAM-1 or 1H4

Produktdetails anti-ICAM1 Antikörper

Spezifität: Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Aufreinigung: Purified by Protein A/G

Immunogen: Raji Burkitt lymphoma cells

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

Known Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: ICAM1

Molekulargewicht: 85-115kDa

Gen-ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling