TNC Antikörper

Produktnummer ABIN6939675

Der Maus Monoklonal Anti-TNC-Antikörper wurde für IHC, IF, FACS und StM validiert. Er ist geeignet, TNC in Proben von Human zu detektieren.

Kurzübersicht für TNC Antikörper (ABIN6939675)

Target: TNC (Tenascin C (TNC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNC Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: SPM319

Produktdetails anti-TNC Antikörper

Spezifität: In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Keine Kreuzreaktivität: Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Human breast carcinoma

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TNC

Target: TNC (Tenascin C (TNC))

Andere Bezeichnung: TNC

Molekulargewicht: 210kDa and 300kDa

Gen-ID: 3371

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development