ABO Antikörper

Produktnummer ABIN6939552

Dieses Anti-ABO-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ABO in ELISA, IF, AGG, H und Coat. Geeignet für Human.

Kurzübersicht für ABO Antikörper (ABIN6939552)

Target: ABO (Histo-blood group A transferase (ABO))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ABO Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Agglutination (AGG), Haemagglutination (H), Coating (Coat)

Klon: HE-14

Produktdetails anti-ABO Antikörper

Spezifität: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.

Aufreinigung: Purified by Protein A/G

Immunogen: Mixture of erythrocytes of blood group A1and glyco protein fraction isolated from the saliva of secretors with blood group A

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: KG1 cells. Human colorectal carcinoma tissues.

Known Application: ELISA (For coating, order Ab without BSA), Agglutination, Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ABO

Target: ABO (Histo-blood group A transferase (ABO))

Andere Bezeichnung: ABO

Gen-ID: 28

UniProt: P16442