GNRHR and LHCGR (AA 1-29), (N-Term) Antikörper

Produktnummer ABIN6939536

Der Maus Monoklonal anti-GNRHR and LHCGR Antikörper wird verwendet zum Nachweis von GNRHR and LHCGR in Proben von Human und Ratte. Er wurde validiert für FACS, IF, IHC und WB.

Kurzübersicht für GNRHR and LHCGR (AA 1-29), (N-Term) Antikörper (ABIN6939536)

Target: GNRHR and LHCGR

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: F1G4

Produktdetails

Bindungsspezifität: AA 1-29, N-Term

Verwendungszweck: GnRH-Receptor / LH-RH Receptor Antibody

Spezifität: Recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations.

Kreuzreaktivität (Details): Predicted to react with Pig, Rabbit.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRH receptor.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, MDA231, T47D cells. Pituitary gland, ovarian or breast cancers.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: GNRHR and LHCGR

Hintergrund:

GnRH receptor, GnRH-R, GNRHR1, gonadotropin-releasing hormone (type 1) receptor 1, GRHR, HH7, leutinizing-releasing hormone receptor, LHRHR, LRHR, luliberin receptor, luteinizing hormone releasing hormone receptor, Type I GnRH receptor

MCF-7, MDA231, ovarian or breast cancers., T47D cells. Pituitary gland

Molekulargewicht: 54-60kDa

Gen-ID: 2798, 3973, 407587468490

UniProt: P30968