FBXO5 Antikörper (AA 1-250)
Kurzübersicht für FBXO5 Antikörper (AA 1-250) (ABIN6939501)
Target
Alle FBXO5 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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                                            Bindungsspezifität
- AA 1-250
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                                            Spezifität
- It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
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                                            Aufreinigung
- Purified by Protein A/G
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                                            Immunogen
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
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                                            Isotyp
- IgG2a kappa
 
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                                            Applikationshinweise
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                        Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma. Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. 
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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                                            Konzentration
- 200 μg/mL
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                                            Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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                                            Konservierungsmittel
- Sodium azide
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                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            Lagerung
- 4 °C,-80 °C
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                                            Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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                                            Haltbarkeit
- 24 months
 
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    - FBXO5 (F-Box Protein 5 (FBXO5))
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                                            Andere Bezeichnung
- FBXO5
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                                            Molekulargewicht
- 56kDa
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                                            Gen-ID
- 26271
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                                            UniProt
- Q9UKT4
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                                            Pathways
- Mitotic G1-G1/S Phases
 Target
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