ESR2 Antikörper (C-Term)

Produktnummer ABIN6939350

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ESR2 in WB, IHC, IF, FACS und StM. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schaf, Schwein, Pferd und Affe.

Kurzübersicht für ESR2 Antikörper (C-Term) (ABIN6939350)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human, Maus, Ratte, Schaf, Schwein, Pferd, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: ERb455

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Purified by Protein A/G

Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: ESR2

Hintergrund: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Molekulargewicht: 53-59kDa

Gen-ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling