Desmoglein 1 Antikörper

Produktnummer ABIN6939264

Dieses Anti-Desmoglein 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Desmoglein 1 in IHC und StM. Geeignet für Human.

Kurzübersicht für Desmoglein 1 Antikörper (ABIN6939264)

Target: Desmoglein 1 (DSG1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: DSG1-1733

Produktdetails anti-Desmoglein 1 Antikörper

Spezifität: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 or A-375 cells. Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Desmoglein 1

Target: Desmoglein 1 (DSG1)

Andere Bezeichnung: DSG1

Molekulargewicht: 150kDa (precursor), 160kDa (mature)

Gen-ID: 1828

UniProt: Q02413

Pathways: Cell-Cell Junction Organization