Desmoglein 1 Antikörper (Intracellular)

Produktnummer ABIN6939262

Dieser Maus Monoklonal Antikörper detektiert spezifisch Desmoglein 1 in WB, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Desmoglein 1 Antikörper (Intracellular) (ABIN6939262)

Target: Desmoglein 1 (DSG1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 18D4

Produktdetails anti-Desmoglein 1 Antikörper

Bindungsspezifität: Intracellular

Verwendungszweck: Desmoglein-1 (DSG1) Antibody

Spezifität: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human desmoglein-1 intracellular domain

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 or A-375 cells. Skin.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL) Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Desmoglein 1

Target: Desmoglein 1 (DSG1)

Andere Bezeichnung: DSG1

Hintergrund:

Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1

A431 or A-375 cells. Human skin.

Molekulargewicht: 150kDa (precursor), 160kDa (mature)

Gen-ID: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization