Rekombinanter Desmin Antikörper

Produktnummer ABIN6939235

Der Kaninchen Monoklonal anti-Desmin Antikörper wird verwendet zum Nachweis von Desmin in Proben von Human, Ratte, Huhn, Hamster, Kaninchen und Meerschweinchen. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter Desmin Antikörper (ABIN6939235)

Target: Desmin (DES)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Huhn, Hamster, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: DES-2960R

Produktdetails anti-Desmin Antikörper

Verwendungszweck: Recombinant Desmin (Muscle Cell Marker) Antibody

Spezifität: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length DES protein. Human Heart, Rat Heart, Hamster Heart, Guinea pig Heart, Rabbit Heart.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: DES

Hintergrund:

CMD1I, CSM1, CSM2, DES, Intermediate filament protein

SJRH30 cells. Uterus or Leiomyosarcoma, Human Heart

Molekulargewicht: 52kDa

Gen-ID: 1674, 594952

UniProt: P17661