Desmin Antikörper

Produktnummer ABIN6939233

Dieses Anti-Desmin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Desmin in WB, IHC, IF, FACS und StM. Geeignet für Human, Ratte, Maus, Huhn und Hamster.

Kurzübersicht für Desmin Antikörper (ABIN6939233)

Target: Desmin (DES)

Reaktivität: Human, Ratte, Maus, Huhn, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: DES-1711

Produktdetails anti-Desmin Antikörper

Spezifität: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human desmin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SJRH30 cells. Uterus or Leiomyosarcoma.

Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: DES

Molekulargewicht: 52kDa

Gen-ID: 1674

UniProt: P17661