Rekombinanter CNN1 Antikörper

Produktnummer ABIN6939165

Dieses Anti-CNN1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CNN1 in FACS, IHC, WB und StM. Geeignet für Human und Ratte.

Kurzübersicht für Rekombinanter CNN1 Antikörper (ABIN6939165)

Target: CNN1 (Calponin 1 (CNN1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CNN1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)

Klon: RCNN1-832

Produktdetails anti-CNN1 Antikörper

Spezifität: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human CNN1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562 cells, Myoepithelial cells in breast ducts.

Known Application: Western Blot (0.5-1 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: CNN1

Molekulargewicht: 34kDa

Gen-ID: 1264

UniProt: P51911