CKB Antikörper

Produktnummer ABIN6939145

Der Maus Monoklonal anti-CKB Antikörper wird verwendet zum Nachweis von CKB in Proben von Human, Ratte, Maus, Meerschweinchen und Hamster. Er wurde validiert für WB und IHC.

Kurzübersicht für CKB Antikörper (ABIN6939145)

Target: CKB (Creatine Kinase, Brain (CKB))

Reaktivität: Human, Ratte, Maus, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CKB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-CKB-2

Produktdetails anti-CKB Antikörper

Verwendungszweck: Creatine Phosphokinase-BB (CK-BB) Antibody

Spezifität: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length CKB protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SH-SY5Y, HEK-293 and Cerebellum.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Andere Bezeichnung: CKB

Hintergrund:

BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211

Y79 or HEK-293T cells. Human cerebellum. Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach or Guinea pig Stomach tissue

Molekulargewicht: 42kDa

Gen-ID: 1152, 173724

UniProt: P12277