CHD4 Antikörper (C-Term)

Produktnummer ABIN6939119

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CHD4 in WB, ELISA, IF, FACS, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Synthetic.

Kurzübersicht für CHD4 Antikörper (C-Term) (ABIN6939119)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reaktivität: Synthetic

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 3F2-4

Produktdetails anti-CHD4 Antikörper

Bindungsspezifität: C-Term

Spezifität: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Kreuzreaktivität (Details): Human, Mouse,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Andere Bezeichnung: CHD4

Hintergrund: ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
Cellular localisation: Nucleus and cytoplasm.

Molekulargewicht: 238kDa

Gen-ID: 1108, 162233

UniProt: Q14839