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CHD4 Antikörper (C-Term)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CHD4 in WB, ELISA, IF, FACS, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Synthetic.
Produktnummer ABIN6939119

Kurzübersicht für CHD4 Antikörper (C-Term) (ABIN6939119)

Target

Alle CHD4 Antikörper anzeigen
CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reaktivität

  • 52
  • 36
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Synthetic

Wirt

  • 36
  • 18
  • 1
Maus

Klonalität

  • 33
  • 22
Monoklonal

Konjugat

  • 36
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CHD4 Antikörper ist unkonjugiert

Applikation

  • 38
  • 24
  • 21
  • 16
  • 11
  • 10
  • 6
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon

3F2-4
  • Bindungsspezifität

    • 12
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Spezifität

    CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

    Kreuzreaktivität (Details)

    Human, Mouse,

    Aufreinigung

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    Immunogen

    Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

    Isotyp

    IgG2a
  • Applikationshinweise

    Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

    Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

    Haltbarkeit

    24 months
  • Target

    CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

    Andere Bezeichnung

    CHD4

    Hintergrund

    ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
    Cellular localisation: Nucleus and cytoplasm.

    Molekulargewicht

    238kDa

    Gen-ID

    1108, 162233

    UniProt

    Q14839
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