Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Occludin Antikörper (AA 282-415)

This anti-Occludin antibody (ABIN6939017) is a Mouse Monoclonal antibody detecting Occludin in WB, IHC (f). Suitable for Human.
Produktnummer ABIN6939017

Quick Overview for Occludin Antikörper (AA 282-415) (ABIN6939017)

Target

Alle Occludin (OCLN) Antikörper anzeigen
Occludin (OCLN)

Reaktivität

  • 107
  • 65
  • 45
  • 34
  • 18
  • 13
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
Human

Wirt

  • 96
  • 19
  • 3
  • 1
Maus

Klonalität

  • 90
  • 29
Monoklonal

Konjugat

  • 60
  • 9
  • 6
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Dieser Occludin Antikörper ist unkonjugiert

Applikation

  • 96
  • 37
  • 32
  • 27
  • 26
  • 26
  • 20
  • 18
  • 15
  • 13
  • 6
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon

OCLN-2181
  • Bindungsspezifität

    • 16
    • 16
    • 11
    • 9
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 282-415

    Spezifität

    Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.

    Kreuzreaktivität (Details)

    Human,

    Aufreinigung

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    Immunogen

    Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

    Isotyp

    IgG1
  • Applikationshinweise

    Positive Control: HepG2 cells. Kidney.

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

    Haltbarkeit

    24 months
  • Target

    Occludin (OCLN)

    Andere Bezeichnung

    OCLN

    Hintergrund

    BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin,Occludin (Marker of Early Blood Brain Barrier Damage)
    Cellular localisation: Cell Surface and Cytoplasmic

    Molekulargewicht

    60-82kDa

    Gen-ID

    100506658, 592605

    UniProt

    Q16625

    Pathways

    Cell-Cell Junction Organization, Hepatitis C
Sie sind hier:
Chat with us!