Occludin Antikörper (AA 282-415)
Quick Overview for Occludin Antikörper (AA 282-415) (ABIN6939017)
Target
Alle Occludin (OCLN) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 282-415
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Spezifität
- Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.
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Kreuzreaktivität (Details)
- Human,
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Aufreinigung
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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Immunogen
- Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
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Isotyp
- IgG1
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Applikationshinweise
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Positive Control: HepG2 cells. Kidney.
Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
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Haltbarkeit
- 24 months
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- Occludin (OCLN)
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Andere Bezeichnung
- OCLN
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Hintergrund
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BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin,Occludin (Marker of Early Blood Brain Barrier Damage)
Cellular localisation: Cell Surface and Cytoplasmic -
Molekulargewicht
- 60-82kDa
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Gen-ID
- 100506658, 592605
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UniProt
- Q16625
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Pathways
- Cell-Cell Junction Organization, Hepatitis C
Target
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