WT1 Antikörper (AA 1-181)

Produktnummer ABIN6938782

Dieses Anti-WT1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von WT1 in IHC (f). Geeignet für Human.

Kurzübersicht für WT1 Antikörper (AA 1-181) (ABIN6938782)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 6F-H2

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 1-181

Spezifität: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Kreuzreaktivität (Details): Human. Rat. Weakly reacts with Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant protein corresponding to residues 1-181 of human WT1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1

Hintergrund: WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1,Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)
Cellular localisation: Nuclear and Cytoplasmic

Molekulargewicht: 47-55kDa

Gen-ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation