TIMP2 Antikörper (AA 56-66)

Produktnummer ABIN6938576

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TIMP2 in WB, IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TIMP2 Antikörper (AA 56-66) (ABIN6938576)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 3A4

Produktdetails anti-TIMP2 Antikörper

Bindungsspezifität: AA 56-66

Spezifität: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Kreuzreaktivität (Details): Human. Mouse. Rat. Guinea Pig. Rabbit. Cow. Zebrafish.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2,TIMP2 (Tissue Inhibitor of Metalloproteinase 2)
Cellular localisation: Cytoplasm and Cell Surface

Molekulargewicht: 21kDa

Gen-ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process