Thrombomodulin Antikörper (AA 69-194)

Produktnummer ABIN6938550

Der Maus Monoklonal Anti-Thrombomodulin-Antikörper wurde für ELISA, Coat, ISt und IHC (f) validiert. Er ist geeignet, Thrombomodulin in Proben von Human zu detektieren.

Kurzübersicht für Thrombomodulin Antikörper (AA 69-194) (ABIN6938550)

Target: Thrombomodulin (THBD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thrombomodulin Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: THBD-1782

Produktdetails anti-Thrombomodulin Antikörper

Bindungsspezifität: AA 69-194

Spezifität: It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP1 cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Thrombomodulin

Target: Thrombomodulin (THBD)

Andere Bezeichnung: THBD

Hintergrund: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM),Thrombomodulin / CD141 (Endothelial Cell Marker)
Cellular localisation: Cell Surface & Cytoplasmic

Molekulargewicht: ~100kDa

Gen-ID: 7056, 2030

UniProt: P07204