Transgelin Antikörper

Produktnummer ABIN6938512

Der Maus Monoklonal anti-Transgelin Antikörper (Klon SPM606) (ABIN6938512) detektiert spezifisch Transgelin in IHC, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Kaninchen, Rind (Kuh) und Schwein.

Kurzübersicht für Transgelin Antikörper (ABIN6938512)

Target: Transgelin (TAGLN)

Reaktivität: Human, Maus, Kaninchen, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transgelin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: SPM606

Produktdetails anti-Transgelin Antikörper

Verwendungszweck: Transgelin (SM22-alpha) Antibody

Spezifität: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Kreuzreaktivität (Details): Human. Cow. Pig. Rabbit. Mouse.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Transgelin

Target: Transgelin (TAGLN)

Andere Bezeichnung: TAGLN

Hintergrund:

2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10

Hs68 or WI 38 cells. Colon carcinoma., U-2 OS

Molekulargewicht: 22kDa

Gen-ID: 6876, 410977

UniProt: Q01995