TAG-72 / CA72.4 Antikörper

Produktnummer ABIN6938501

Dieser Maus Monoklonal Antikörper detektiert spezifisch TAG-72 / CA72.4 in FACS, IF und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Hund, Rind (Kuh), Ratte und Hamster.

Kurzübersicht für TAG-72 / CA72.4 Antikörper (ABIN6938501)

Target: TAG-72 / CA72.4

Reaktivität: Human, Hund, Rind (Kuh), Ratte, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: B72-3

Produktdetails

Verwendungszweck: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Antibody

Spezifität: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells, Breast or lung carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: TAG-72 / CA72.4

Andere Bezeichnung: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)

Hintergrund:

CA72.4, Tumor associated glycoprotein 72

Breast or lung carcinoma., Jurkat cells

Molekulargewicht: 220kDa

Gen-ID: 182875