TAG-72 / CA72.4 Antikörper

Produktnummer ABIN6938501

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in FACS, IF, IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Hund, Rind (Kuh), Ratte und Hamster.

Kurzübersicht für TAG-72 / CA72.4 Antikörper (ABIN6938501)

Target: TAG-72 / CA72.4

Reaktivität: Human, Hund, Rind (Kuh), Ratte, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: B72-3

Produktdetails

Spezifität: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Aufreinigung: Purified by Protein A/G

Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells, Breast or lung carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Antigendetails

Target: TAG-72 / CA72.4

Andere Bezeichnung: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)

Molekulargewicht: 220kDa

Gen-ID: 182875