EpCAM Antikörper (AA 20-60)

Produktnummer ABIN6938490

Der Maus Monoklonal Anti-EpCAM-Antikörper wurde für WB, FACS, IF, IHC und IHC (f) validiert. Er ist geeignet, EpCAM in Proben von Human zu detektieren.

Kurzübersicht für EpCAM Antikörper (AA 20-60) (ABIN6938490)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: EGP40-826

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 20-60

Spezifität: Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Mouse, Cat, Dog or Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HT29 or SK-OV-3 cells (FACS/IF). Breast carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1),Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)
Cellular localisation: Cell surface

Molekulargewicht: 40-43kDa

Gen-ID: 4072, 542050

UniProt: P16422