EpCAM Antikörper (Extracellular Domain)

Produktnummer ABIN6938478

Der Maus Monoklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Human, Cat, Hund und Meerschweinchen. Er wurde validiert für WB, IHC, IF und ELISA.

Kurzübersicht für EpCAM Antikörper (Extracellular Domain) (ABIN6938478)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human, Cat, Hund, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Klon: EGP40-1384

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 100-224, Extracellular Domain

Verwendungszweck: Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

Spezifität: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Kreuzreaktivität (Details): Human. Dog. Cat.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (0.5-1 μg/million cells),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 orHT29 cells. Human lung or ovariancarcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

MCF-7 orHT29 cells. Human lung or ovariancarcinoma (IHC). Human Intestine, Guinea pig Lung or Guinea pig Intestine

Molekulargewicht: 40-43kDa

Gen-ID: 4072, 542050

UniProt: P16422