Rekombinanter CD43 Antikörper
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- Target Alle CD43 (SPN) Antikörper anzeigen
- CD43 (SPN) (Sialophorin (SPN))
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD43 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called b as opposed to a for rSPN/839. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human CD43 protein
- Klon
- RSPN-839
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product SPN Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells. Paracortex in a tonsil or a reactive lymph node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- CD43 (SPN) (Sialophorin (SPN))
- Andere Bezeichnung
- SPN (SPN Produkte)
- Synonyme
- CD43 antikoerper, GALGP antikoerper, GPL115 antikoerper, LSN antikoerper, A630014B01Rik antikoerper, Cd43 antikoerper, Galgp antikoerper, Ly-48 antikoerper, Ly48 antikoerper, Lsn antikoerper, Lsn1 antikoerper, gpL115 antikoerper, sialophorin antikoerper, SPN antikoerper, Spn antikoerper
- Hintergrund
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Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN,CD43 (T-Cell Marker)
Cellular localisation: Cell Surface - Molekulargewicht
- 95, 115, or 135kDa
- Gen-ID
- 6693, 632188
- UniProt
- P16150
- Pathways
- Regulation of Leukocyte Mediated Immunity
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