Rekombinanter SOX2 Antikörper (AA 176-305)

Produktnummer ABIN6938412

Der Maus Monoklonal anti-SOX2 Antikörper wird verwendet zum Nachweis von SOX2 in Proben von Human und Maus. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter SOX2 Antikörper (AA 176-305) (ABIN6938412)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: RSOX2-1791

Produktdetails anti-SOX2 Antikörper

Bindungsspezifität: AA 176-305

Verwendungszweck: Recombinant SOX2 (Embryonic Stem Cell Marker) Antibody

Spezifität: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Kreuzreaktivität (Details): Human. Mouse.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed, Not suitable for frozen tissues) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, NCCIT cells. Human lung or cervical carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Andere Bezeichnung: SOX2

Hintergrund:

ANOP3, Delta EF2a, MCOPS3 (Microphthalmia Syndromic type 3), SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb

A549, NCCIT cells. Human lung or cervical carcinoma.

Molekulargewicht: 34kDa

Gen-ID: 6657, 518438

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis