Rekombinanter SOX2 Antikörper (AA 176-305)

Produktnummer ABIN6938412

Der Maus Monoklonal Anti-SOX2-Antikörper wurde für IHC (f) validiert. Er ist geeignet, SOX2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter SOX2 Antikörper (AA 176-305) (ABIN6938412)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RSOX2-1791

Produktdetails anti-SOX2 Antikörper

Bindungsspezifität: AA 176-305

Spezifität: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Kreuzreaktivität (Details): Human. Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed, Not suitable for frozen tissues) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, NCCIT cells. Human lung or cervical carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Andere Bezeichnung: SOX2

Hintergrund: ANOP3, Delta EF2a, MCOPS3 (Microphthalmia Syndromic type 3), SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb,SOX2 (Embryonic Stem Cell Marker)
Cellular localisation: Nuclear

Molekulargewicht: 34kDa

Gen-ID: 6657, 518438

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis