Proliferation Marker Antikörper

Produktnummer ABIN6938249

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IHC, WB, IF und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Proliferation Marker Antikörper (ABIN6938249)

Target: Proliferation Marker

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)

Klon: JC1

Produktdetails

Aufreinigung: Purified by Protein A/G

Immunogen: Pokeweed-stimulated lymphocytes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells (IF). Human lymph node (IHC).

Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Antigendetails

Target: Proliferation Marker

Hintergrund: JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Molekulargewicht: 212kDa and 123kDa