PDLIM1 Antikörper

Produktnummer ABIN6938142

Der Maus Monoklonal anti-PDLIM1 Antikörper wird verwendet zum Nachweis von PDLIM1 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für PDLIM1 Antikörper (ABIN6938142)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDLIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: CPTC-PDLIM1-1

Produktdetails anti-PDLIM1 Antikörper

Verwendungszweck: PDZ and LIM Domain 1 Antibody

Spezifität: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length PDLIM1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Andere Bezeichnung: PDLIM1

Hintergrund:

C terminal LIM domain protein 1, carboxy terminal LIM domain protein 1, CLIM1, CLP36, Elfin, Enigma antibody, Enigma homolog antibody, hCLIM1, PDLIM1, PDZ and LIM domain 1

Placenta, colon or spleen., HepG2 cells and extracts from Jurkat. Human heart

Molekulargewicht: 36kDa

Gen-ID: 9124, 368525

UniProt: O00151