PAX8 Antikörper (AA 60-261)

Produktnummer ABIN6938127

Produktdetails anti-PAX8 Antikörper

Target: PAX8 (Paired Box 8 (PAX8))

Bindungsspezifität: AA 60-261

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Kreuzreaktivität (Details): Human. Dog.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 60-261) of human PAX8 protein (exact sequence is proprietary)

Klon: PAX8-1492

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Renal Cell Carcinoma (RCC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Andere Bezeichnung: PAX8 (PAX8 Produkte)

Synonyme: PAX8 antikoerper, XPax-8 antikoerper, XPax8 antikoerper, pax-8 antikoerper, PAX-8 antikoerper, Pax-8 antikoerper, paired box 8 antikoerper, paired box 8 L homeolog antikoerper, PAX8 antikoerper, pax8 antikoerper, Pax8 antikoerper, pax8.L antikoerper

Hintergrund: Paired box 8, Paired box gene 8, paired box homeotic gene 8, Paired box protein Pax-8, Paired domain gene 8, PAX8,PAX8 (Renal Cell Marker)
Cellular localisation: Nuclear

Molekulargewicht: 62kDa

Gen-ID: 7849, 469728

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation