MUC2 Antikörper

Produktnummer ABIN6937947

Der Maus Monoklonal Anti-MUC2-Antikörper wurde für IHC, IF, FACS und IHC (f) validiert. Er ist geeignet, MUC2 in Proben von Human zu detektieren.

Kurzübersicht für MUC2 Antikörper (ABIN6937947)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: MLP-842

Produktdetails anti-MUC2 Antikörper

Spezifität: Recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LS174T cells. Human small intestine (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Andere Bezeichnung: MUC2

Hintergrund: Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC,MUC2 (Mucin 2)
Cellular localisation: Cytoplasmic and cell surface

Molekulargewicht: 520kDa

Gen-ID: 4583, 315

UniProt: Q02817