MUC1 Antikörper

Produktnummer ABIN6937921

Dieses Anti-MUC1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MUC1 in WB, FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für MUC1 Antikörper (ABIN6937921)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: VU-4H5

Produktdetails anti-MUC1 Antikörper

Spezifität: MAb VU-4H5 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of MAb VU4H5 is APDTR as established with 'epitope fingerprinting'. VU-4H5 preferentially binds to under-glycosylated 'tumor' MUC1. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3-COOH, conjugated to bovine serum albumin

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC1

Hintergrund: Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen,MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)
Cellular localisation: Cytoplasmic and cell surface

Molekulargewicht: 265-400kDa

Gen-ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling