Rekombinanter MTAP Antikörper (AA 97-196)

Produktnummer ABIN6937918

Produktdetails anti-MTAP Antikörper

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 97-196

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MTAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Klon: MTAP-3137R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, A431, HeLa or MCF-7 cells. Human kidney tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Andere Bezeichnung: MTAP (MTAP Produkte)

Synonyme: BDMF antikoerper, DMSFH antikoerper, DMSMFH antikoerper, LGMBF antikoerper, MSAP antikoerper, c86fus antikoerper, Cc1-6 antikoerper, 1300019I21Rik antikoerper, MTAPase antikoerper, zgc:66012 antikoerper, mtap antikoerper, methylthioadenosine phosphorylase antikoerper, 5'-methylthioadenosine phosphorylase antikoerper, 6-oxopurine nucleoside phosphorylase antikoerper, methylthioadenosine phosphorylase L homeolog antikoerper, MTAP antikoerper, Mtap antikoerper, mtaP antikoerper, MMAH_RS04260 antikoerper, TAGG_RS05050 antikoerper, Toce_0364 antikoerper, VDIS_RS03165 antikoerper, MFER_RS02485 antikoerper, Intca_0886 antikoerper, DESMU_RS05370 antikoerper, Dester_1490 antikoerper, ARCVE_RS05445 antikoerper, Mahau_1873 antikoerper, MZHIL_RS01440 antikoerper, mtap antikoerper, mtap.L antikoerper

Hintergrund: BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAPase, S-methyl-5''-thioadenosine phosphorylase,MTAP (Tumor Suppressor Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 31kDa

Gen-ID: 4507, 193268

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process