Rekombinanter MTAP Antikörper (AA 97-196)

Produktnummer ABIN6937917

Der Maus Monoklonal Anti-MTAP-Antikörper wurde für IHC, ISt und IHC (f) validiert. Er ist geeignet, MTAP in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter MTAP Antikörper (AA 97-196) (ABIN6937917)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MTAP Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RMTAP-1813

Produktdetails anti-MTAP Antikörper

Bindungsspezifität: AA 97-196

Spezifität: Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, A431, HeLa or MCF-7 cells. Human kidney tissues (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Andere Bezeichnung: MTAP

Hintergrund: BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAPase, S-methyl-5''-thioadenosine phosphorylase,MTAP (Tumor Suppressor Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 31kDa

Gen-ID: 4507, 193268

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process