MLANA & SILV Antikörper
Kurzübersicht für MLANA & SILV Antikörper (ABIN6937867)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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Kreuzreaktivität (Details)
- Human.
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Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
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Isotyp
- IgG1 kappa
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anti-MLANA & SILV antibody
Reaktivität: Human IHC, ISt, StM Wirt: Maus Monoclonal DT101-BC199-HMB45 unconjugated
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Applikationshinweise
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Konservierungsmittel
- Azide free
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Haltbarkeit
- 24 months
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