MAML3 Antikörper

Produktnummer ABIN6937803

Der Maus Monoklonal Anti-MAML3-Antikörper wurde für ISt und IHC (f) validiert. Er ist geeignet, MAML3 in Proben von Human zu detektieren.

Kurzübersicht für MAML3 Antikörper (ABIN6937803)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML3 Antikörper ist unkonjugiert

Applikation: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: MAML3-1303

Produktdetails anti-MAML3 Antikörper

Spezifität: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human MAML3 protein fragment

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Andere Bezeichnung: MAML3

Hintergrund: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3,MAML3 (Mastermind Like Transcriptional Coactivator 3)
Cellular localisation: Cytoplasmic

Molekulargewicht: 150-170kDa

Gen-ID: 55534, 586165

UniProt: Q96JK9