Rekombinanter LGALS1/Galectin 1 Antikörper (AA 12-108)

Produktnummer ABIN6937775

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch LGALS1/Galectin 1 in WB, ELISA, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter LGALS1/Galectin 1 Antikörper (AA 12-108) (ABIN6937775)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser LGALS1/Galectin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: GAL1-2499R

Produktdetails anti-LGALS1/Galectin 1 Antikörper

Bindungsspezifität: AA 12-108

Spezifität: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 12-108) of human Galectin-1 protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3, HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Andere Bezeichnung: LGALS1

Hintergrund: 14 kDa laminin-binding protein, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, HLBP14, HPL, Lactose-binding lectin 1, Lect14, Lectin galactoside-binding soluble 1, LGALS1, MAPK activating protein MP12, S-Lac lectin 1,Galectin-1 / Human Placental Lactogen (hPL)
Cellular localisation: Secreted (extracellular matrix)

Molekulargewicht: 14kDa

Gen-ID: 3956, 445351

UniProt: P09382

Pathways: Carbohydrate Homeostasis