KRT4 Antikörper (AA 181-292)

Produktnummer ABIN6937710

Der Maus Monoklonal Anti-KRT4-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, KRT4 in Proben von Human zu detektieren.

Kurzübersicht für KRT4 Antikörper (AA 181-292) (ABIN6937710)

Target: KRT4 (Keratin 4 (KRT4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: KRT4-2804

Produktdetails anti-KRT4 Antikörper

Bindungsspezifität: AA 181-292

Spezifität: Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu KRT4

Target: KRT4 (Keratin 4 (KRT4))

Andere Bezeichnung: KRT4

Hintergrund: CK4, CYK4, Cytokeratin 4, Keratin 4, Keratin type II cytoskeletal 4, KRT4,Cytokeratin 4 (KRT4)
Cellular localisation: Cytoplasmic and Cell Surface

Molekulargewicht: 67kDa

Gen-ID: 3851, 654610, 706527

UniProt: P19013