Rekombinanter KRT20 Antikörper (AA 196-323)

Produktnummer ABIN6937708

Dieses Anti-KRT20-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von KRT20 in WB, IHC und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter KRT20 Antikörper (AA 196-323) (ABIN6937708)

Target: KRT20 (Keratin 20 (KRT20))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser KRT20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: KRT20-3129R

Produktdetails anti-KRT20 Antikörper

Bindungsspezifität: AA 196-323

Spezifität: This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HT29 cell lysate (WB). Human colon carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu KRT20

Target: KRT20 (Keratin 20 (KRT20))

Andere Bezeichnung: KRT20

Hintergrund: CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 46kDa

Gen-ID: 54474, 84905

UniProt: P35900