Cytokeratin 19 Antikörper

Produktnummer ABIN6937692

Der Maus Monoklonal Anti-Cytokeratin 19-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, Cytokeratin 19 in Proben von Human zu detektieren.

Kurzübersicht für Cytokeratin 19 Antikörper (ABIN6937692)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: A53-B-A2-26

Produktdetails anti-Cytokeratin 19 Antikörper

Spezifität: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Keine Kreuzreaktivität: Frettchen, Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Rat or Ferret.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human breast cancer MCF-7 cells

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa or Hep-G2 cells, Breast cancer tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: KRT19

Hintergrund: K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19,Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 40kDa

Gen-ID: 3880, 654568

UniProt: P08727