Rekombinanter MHC Class II HLA-DP/DQ/DR Antikörper

Produktnummer ABIN6937398

Der Maus Monoklonal anti-MHC Class II HLA-DP/DQ/DR Antikörper wird verwendet zum Nachweis von MHC Class II HLA-DP/DQ/DR in Proben von Human. Er wurde validiert für FACS, IHC, WB und IF.

Kurzübersicht für Rekombinanter MHC Class II HLA-DP/DQ/DR Antikörper (ABIN6937398)

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II HLA-DP/DQ/DR Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Klon: RHLA-Pan-3475

Produktdetails anti-MHC Class II HLA-DP/DQ/DR Antikörper

Verwendungszweck: Recombinant HLA-Pan (MHC II) Antibody

Spezifität: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji cells. Human tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MHC Class II HLA-DP/DQ/DR

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Andere Bezeichnung: HLA-DP, HLA-DQ, HLA-DR

Hintergrund:

HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Daudi, Raji cells. Human tonsil or lymph node. Human Intestine, Human Lung

Molekulargewicht: 33-35kDa

UniProt: P01908, P01909

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response