GPI Antikörper

Produktnummer ABIN6937345

Der Maus Monoklonal anti-GPI Antikörper (Klon CPTC-GPI-1) (ABIN6937345) detektiert spezifisch GPI in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GPI Antikörper (ABIN6937345)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GPI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: CPTC-GPI-1

Produktdetails anti-GPI Antikörper

Verwendungszweck: Glucose 6-Phosphate Isomerase Antibody

Spezifität: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length GPI protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Andere Bezeichnung: GPI

Hintergrund:

AMF, aurocrine motility factor, EC 5.2.1.9, GNPI, Gpi1, hexose monophosphate isomerase, neuroleukin, NLK, oxoisomerase, PGI, PHI, phosphoglucose isomerase, phosphosaccharomutase, SA-36, sperm antigen 36

HepG2 and A549 cells. Human Kidney or Brain, MCF-7, pancreas or thyroid carcinoma., PC3, T47D

Molekulargewicht: 55kDa

Gen-ID: 2821, 466471

UniProt: P06744