GPI Antikörper

Produktnummer ABIN6937345

Dieses Anti-GPI-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GPI in WB, IF und IHC (f). Geeignet für Human.

Kurzübersicht für GPI Antikörper (ABIN6937345)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GPI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CPTC-GPI-1

Produktdetails anti-GPI Antikörper

Spezifität: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length GPI protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Andere Bezeichnung: GPI

Hintergrund: AMF, aurocrine motility factor, EC 5.2.1.9, GNPI, Gpi1, hexose monophosphate isomerase, neuroleukin, NLK, oxoisomerase, PGI, PHI, phosphoglucose isomerase, phosphosaccharomutase, SA-36, sperm antigen 36,Glucose 6-Phosphate Isomerase
Cellular localisation: Cytoplasm. Secreted.

Molekulargewicht: 55kDa

Gen-ID: 2821, 466471

UniProt: P06744