Rekombinanter FTL Antikörper (AA 38-165)

Produktnummer ABIN6937277

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FTL in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter FTL Antikörper (AA 38-165) (ABIN6937277)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RFTL-1388

Produktdetails anti-FTL Antikörper

Bindungsspezifität: AA 38-165

Spezifität: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 38-165) of human FTL protein (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Kidney, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: FTL

Hintergrund: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3,Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 19-25kDa

Gen-ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis