Rekombinanter FTL Antikörper (AA 38-165)

Produktnummer ABIN6937272

Der Kaninchen Monoklonal anti-FTL Antikörper wird verwendet zum Nachweis von FTL in Proben von Human, Maus, Ratte, Kaninchen, Meerschweinchen und Hamster. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter FTL Antikörper (AA 38-165) (ABIN6937272)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Kaninchen, Meerschweinchen, Hamster

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser FTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: FTL-2338R

Produktdetails anti-FTL Antikörper

Bindungsspezifität: AA 38-165

Verwendungszweck: Recombinant Ferritin, Light Chain (FTL) (Microglia Marker) Antibody

Spezifität: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human FTL protein fragment (around aa 38-165) (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Kidney, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: FTL

Hintergrund:

Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Cerebellum or Testis., HeLa, HePG2, HL-60 or 293T cells. Pancreas, Kidney, Liver, Human Brain, Human Kidney, Human Liver, Human Lung, A431, Spleen

Molekulargewicht: 19-25kDa

Gen-ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis