Rekombinanter Filaggrin Antikörper (AA 198-288)

Produktnummer ABIN6937235

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Filaggrin in IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Filaggrin Antikörper (AA 198-288) (ABIN6937235)

Target: Filaggrin (FLG)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Filaggrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RFLG-1561

Produktdetails anti-Filaggrin Antikörper

Bindungsspezifität: AA 198-288

Spezifität: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: U-251-MG cells. Skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Filaggrin

Target: Filaggrin (FLG)

Andere Bezeichnung: FLG

Hintergrund: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin,Filaggrin (Keratinocyte Differentiation Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 26-45kDa (Processed), 350kDa (Profilaggrin)

Gen-ID: 2312, 654510

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance