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FBXO5 Antikörper (AA 1-250)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FBXO5 in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN6937217

Kurzübersicht für FBXO5 Antikörper (AA 1-250) (ABIN6937217)

Target

Alle FBXO5 Antikörper anzeigen
FBXO5 (F-Box Protein 5 (FBXO5))

Reaktivität

  • 41
  • 9
  • 9
  • 2
  • 2
  • 1
Human

Wirt

  • 24
  • 17
Maus

Klonalität

  • 25
  • 16
Monoklonal

Konjugat

  • 22
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser FBXO5 Antikörper ist unkonjugiert

Applikation

  • 33
  • 15
  • 12
  • 10
  • 8
  • 7
  • 5
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon

EMI1-1176
  • Bindungsspezifität

    • 11
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 1-250

    Spezifität

    It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

    Kreuzreaktivität (Details)

    Human.

    Aufreinigung

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogen

    Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Konservierungsmittel

    Azide free

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Haltbarkeit

    24 months
  • Target

    FBXO5 (F-Box Protein 5 (FBXO5))

    Andere Bezeichnung

    FBXO5

    Hintergrund

    Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31,EMI1 (Early Mitotic Inhibitor-1)
    Cellular localisation: Nuclear

    Molekulargewicht

    56kDa

    Gen-ID

    26271, 520506

    UniProt

    Q9UKT4

    Pathways

    Mitotic G1-G1/S Phases
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