FBXO5 Antikörper (AA 1-250)
Kurzübersicht für FBXO5 Antikörper (AA 1-250) (ABIN6937217)
Target
Alle FBXO5 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 1-250
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Spezifität
- It recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
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Kreuzreaktivität (Details)
- Human.
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Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
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Isotyp
- IgG2a kappa
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Applikationshinweise
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Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Konservierungsmittel
- Azide free
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Haltbarkeit
- 24 months
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- FBXO5 (F-Box Protein 5 (FBXO5))
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Andere Bezeichnung
- FBXO5
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Hintergrund
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Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31,EMI1 (Early Mitotic Inhibitor-1)
Cellular localisation: Nuclear -
Molekulargewicht
- 56kDa
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Gen-ID
- 26271, 520506
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UniProt
- Q9UKT4
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Pathways
- Mitotic G1-G1/S Phases
Target
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