Ezrin Antikörper

Produktnummer ABIN6937202

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Ezrin in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Ezrin Antikörper (ABIN6937202)

Target: Ezrin (EZR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ezrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CPTC-Ezrin-1

Produktdetails anti-Ezrin Antikörper

Spezifität: Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70 % homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma.

Kreuzreaktivität (Details): Human. Others-not tested.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length Ezrin protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blotting (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SKBr3, HeLa, HEK293, MCF-7, Jurkat, A431, MCF-7. Cerebral cortex and hippocampus.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Ezrin

Target: Ezrin (EZR)

Andere Bezeichnung: Ezrin

Hintergrund: Villin-2, CVIL, Epididymis secretory protein Li 105, EZR, p81, VIL2, Cytovillin, Cytovillin 2, HEL S 105, DKFZp762H157,Ezrin / p81
Cellular localisation: Cell Surface

Molekulargewicht: 87kDa

Gen-ID: 7430, 487027

UniProt: P15311

Pathways: Maintenance of Protein Location